Synthetic seed production from somatic embryos of Pinus radiata

Felipe Aquea, María Josefina Poupin, José Tomás Matus, Marlene Gebauer, Consuelo Medina, Patricio Arce-Johnson

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

25 Citas (Scopus)

Resumen

Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.

Idioma originalInglés
Páginas (desde-hasta)1847-1852
Número de páginas6
PublicaciónBiotechnology Letters
Volumen30
N.º10
DOI
EstadoPublicada - oct. 2008

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