TY - JOUR
T1 - Impact of copper on the abundance and diversity of sulfate-reducing prokaryotes in two chilean marine sediments
AU - Besaury, Ludovic
AU - Ouddane, Baghdad
AU - Pavissich, Juan Pablo
AU - Dubrulle-Brunaud, Carole
AU - González, Bernardo
AU - Quillet, Laurent
N1 - Funding Information:
This work was supported by the International Collaborative ECOS-CONICYT program (no. C06B05) and the ECCO French research program “Misechicui”. We thank Dilys Moscato for help with the English.
PY - 2012/10
Y1 - 2012/10
N2 - We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene β-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed.
AB - We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene β-subunit (dsrB) and showed that SRPs are sensitive to high copper concentrations, as the mean number of SRPs all along the contaminated sediment was two orders of magnitude lower than in the reference sediment. SRP diversity was analyzed by using the dsrB-sequences-based PCR-DGGE method and constructing gene libraries for dsrB-sequences. Surprisingly, the diversity was comparable in both sediments, with dsrB sequences belonging to Desulfobacteraceae, Syntrophobacteraceae, and Desulfobulbaceae, SRP families previously described in marine sediments, and to a deep branching dsrAB lineage. The hypothesis of the presence of horizontal transfer of copper resistance genes in the microbial population of the polluted sediment is discussed.
KW - Copper contamination
KW - DGGE
KW - Marine sediment
KW - Phylogenetic analysis
KW - Real-time PCR
KW - Sulfate-reducing prokaryotes
UR - https://www.scopus.com/pages/publications/84866746055
U2 - 10.1016/j.marpolbul.2012.07.042
DO - 10.1016/j.marpolbul.2012.07.042
M3 - Article
C2 - 22921896
AN - SCOPUS:84866746055
SN - 0025-326X
VL - 64
SP - 2135
EP - 2145
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 10
ER -