Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante la técnica FISH

Fluorescent in situ hybridization (FISH) was employed for the identification of the main bacterial populations involved in nitrogen removal process. In one of the reactors, (SBRN) biomass in form of aerobic granules carrying on the nitrification process was developed. In the other reactor, (SBRA), biomass was enriched in microorganisms performing anaerobic ammonium oxidation (Anammox). With this technique it was possible to identify bacteria belonging to genus Nitrosomonas as dominant ammonia oxidizers, and Nitrobacter and Nitrospira as nitrite oxidizers in SBRN. Candidatus Kuenenia stuttgartiensis were detected as the main Anammox bacteria in SBRA.