1. 1. The binding of RNA polymerase from the extreme thermophile T. thermophilus HB8 to plasmid pBR322 was measured by electron microscopy. 2. 2. DNA-protein complexes were prepared at 35 and 60°C. At both temperatures the enzyme binds strongly to sites which coincide with promoters P1, P2 P3 and P4 present in pBR322. At 60°C, an additional binding site appears, which is located between P3 and P4. 3. 3. There is a high degree of correlation between RNA polymerase binding sites and the location of A-T rich regions on pBR322 DNA.
|Número de páginas||3|
|Publicación||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Estado||Publicada - 1985|