@article{696e1bd2aded43e497e20018ff5d68f8,
title = "Wall mechanics and exocytosis define the shape of growth domains in fission yeast",
abstract = "The amazing structural variety of cells is matched only by their functional diversity, and reflects the complex interplay between biochemical and mechanical regulation. How both regulatory layers generate specifically shaped cellular domains is not fully understood. Here, we report how cell growth domains are shaped in fission yeast. Based on quantitative analysis of cell wall expansion and elasticity, we develop a model for how mechanics and cell wall assembly interact and use it to look for factors underpinning growth domain morphogenesis. Surprisingly, we find that neither the global cell shape regulators Cdc42-Scd1-Scd2 nor the major cell wall synthesis regulators Bgs1-Bgs4-Rgf1 are reliable predictors of growth domain geometry. Instead, their geometry can be defined by cell wall mechanics and the cortical localization pattern of the exocytic factors Sec6-Syb1-Exo70. Forceful re-directioning of exocytic vesicle fusion to broader cortical areas induces proportional shape changes to growth domains, demonstrating that both features are causally linked.",
author = "Abenza, {Juan F.} and Etienne Couturier and James Dodgson and Johanna Dickmann and Anatole Chessel and Jacques Dumais and {Carazo Salas}, {Rafael E.}",
note = "Funding Information: We thank P. Nurse and J. Hayles for help in the initial phases of this work, A. Csik{\'a}sz-Nagy, F. Vaggi, T. Haack, P. Cicuta, D. Bray and the Carazo-Salas group for help and comments, A. Sossick and N. Lawrence for assistance with imaging, J.C. Ribas, Y. S{\'a}nchez, S. Martin, M. Sato and M. Edamatsu for S. pombe strains, and D. Bray, P. Cicuta, M. Geymonat, J. Lawson, E. Rojas and P. P{\'e}rez for critical reading of the manuscript. This work was supported by an European Research Council (ERC) Starting Researcher Investigator Grant (R.E.C.S.; SYSGRO), a Ram{\'o}n Areces Foundation Postdoctoral Fellowship (J.F.A.), a Herchel Smith Postdoctoral Fellowship (J.F.A.), a Human Frontier Science Program (HFSP) Young Investigator Grant (R.E.C.S.; HFSP RGY0066/2009-C), a Biological Sciences Research Council (BBSRC) Responsive Mode grant (R.E.C.S.; BB/K006320/1), an Isaac Newton Trust research grant (R.E.C.S.; 10.44(n)), a Fondecyt regular grant (J.Du.; 1130129), a Fondecyt postdoctoral fellowship (E.C.; 3120105) and a Baden-W{\"u}rttemberg-STIPENDIUM (J.Di.). Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",
year = "2015",
month = oct,
day = "12",
doi = "10.1038/ncomms9400",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
}