TY - JOUR
T1 - Transient concentrations of NaCl affect the PHA accumulation in mixed microbial culture
AU - Palmeiro-Sánchez, T.
AU - Fra-Vázquez, A.
AU - Rey-Martínez, N.
AU - Campos, J. L.
AU - Mosquera-Corral, A.
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/4/5
Y1 - 2016/4/5
N2 - The present study explores the feasibility of the accumulation of polyhydroxyalkanoates (PHAs) under the presence of transient concentrations of added sodium chloride, by means of a mixed microbial culture (MMC). This culture was enriched on a mixture of volatile fatty acids (VFAs) containing 0.8g Na+/L as NaOH. This MMC presented a maximum PHA accumulation capacity of 53wt% with 27Cmol% HV. Accumulation experiments performed with added NaCl at concentrations of 7, 13 and 20g/L shown that this salt provoked a decrease of the biomass PHA production rate, with an IC50 value close to 7gNaCl/L. The accumulated PHA was lower than the corresponding value of the assay without the addition of salt. Furthermore, the composition of the biopolymer, in terms of HB:HV ratio, changed from 2.71 to 6.37Cmol/Cmol, which means a HV decrease between 27 and 14Cmol%. Summarizing, the PHA accumulation by a MMC non-adapted to saline conditions affected the polymer composition and lead to lower production yields and rates than in absence of added NaCl.
AB - The present study explores the feasibility of the accumulation of polyhydroxyalkanoates (PHAs) under the presence of transient concentrations of added sodium chloride, by means of a mixed microbial culture (MMC). This culture was enriched on a mixture of volatile fatty acids (VFAs) containing 0.8g Na+/L as NaOH. This MMC presented a maximum PHA accumulation capacity of 53wt% with 27Cmol% HV. Accumulation experiments performed with added NaCl at concentrations of 7, 13 and 20g/L shown that this salt provoked a decrease of the biomass PHA production rate, with an IC50 value close to 7gNaCl/L. The accumulated PHA was lower than the corresponding value of the assay without the addition of salt. Furthermore, the composition of the biopolymer, in terms of HB:HV ratio, changed from 2.71 to 6.37Cmol/Cmol, which means a HV decrease between 27 and 14Cmol%. Summarizing, the PHA accumulation by a MMC non-adapted to saline conditions affected the polymer composition and lead to lower production yields and rates than in absence of added NaCl.
KW - Inhibition
KW - Microbial mixed culture
KW - Polyhydroxyalkanoates
KW - Sodium chloride
KW - Volatile fatty acids
UR - http://www.scopus.com/inward/record.url?scp=84954146998&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2015.12.032
DO - 10.1016/j.jhazmat.2015.12.032
M3 - Article
C2 - 26780589
AN - SCOPUS:84954146998
SN - 0304-3894
VL - 306
SP - 332
EP - 339
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -