Transient concentrations of NaCl affect the PHA accumulation in mixed microbial culture

T. Palmeiro-Sánchez, A. Fra-Vázquez, N. Rey-Martínez, J. L. Campos, A. Mosquera-Corral

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27 Scopus citations

Abstract

The present study explores the feasibility of the accumulation of polyhydroxyalkanoates (PHAs) under the presence of transient concentrations of added sodium chloride, by means of a mixed microbial culture (MMC). This culture was enriched on a mixture of volatile fatty acids (VFAs) containing 0.8g Na+/L as NaOH. This MMC presented a maximum PHA accumulation capacity of 53wt% with 27Cmol% HV. Accumulation experiments performed with added NaCl at concentrations of 7, 13 and 20g/L shown that this salt provoked a decrease of the biomass PHA production rate, with an IC50 value close to 7gNaCl/L. The accumulated PHA was lower than the corresponding value of the assay without the addition of salt. Furthermore, the composition of the biopolymer, in terms of HB:HV ratio, changed from 2.71 to 6.37Cmol/Cmol, which means a HV decrease between 27 and 14Cmol%. Summarizing, the PHA accumulation by a MMC non-adapted to saline conditions affected the polymer composition and lead to lower production yields and rates than in absence of added NaCl.

Original languageEnglish
Pages (from-to)332-339
Number of pages8
JournalJournal of Hazardous Materials
Volume306
DOIs
StatePublished - 5 Apr 2016
Externally publishedYes

Keywords

  • Inhibition
  • Microbial mixed culture
  • Polyhydroxyalkanoates
  • Sodium chloride
  • Volatile fatty acids

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