Synthetic seed production from somatic embryos of Pinus radiata

Felipe Aquea; María Josefina Poupin; José Tomás Matus; Marlene Gebauer; Consuelo Medina; Patricio Arce-Johnson

doi:10.1007/s10529-008-9754-x

Synthetic seed production from somatic embryos of Pinus radiata

Felipe Aquea, María Josefina Poupin, José Tomás Matus, Marlene Gebauer, Consuelo Medina, Patricio Arce-Johnson

Faculty of Engineering and Science

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.

Original language	English
Pages (from-to)	1847-1852
Number of pages	6
Journal	Biotechnology Letters
Volume	30
Issue number	10
DOIs	https://doi.org/10.1007/s10529-008-9754-x
State	Published - Oct 2008

Keywords

Conifer embryogenesis
Pinus radiata
Somatic embryo
Suspension culture
Synthetic seed

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title = "Synthetic seed production from somatic embryos of Pinus radiata",

abstract = "Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.",

keywords = "Conifer embryogenesis, Pinus radiata, Somatic embryo, Suspension culture, Synthetic seed",

author = "Felipe Aquea and Poupin, {Mar{\'i}a Josefina} and Matus, {Jos{\'e} Tom{\'a}s} and Marlene Gebauer and Consuelo Medina and Patricio Arce-Johnson",

note = "Funding Information: Acknowledgments F.A. and M.J.P was supported by a PhD fellowship from CONICYT. F.A was supported VRAID fellowship from the Pontificia Universidad Cat{\'o}lica de Chile. J.T.M was supported by MECESUP.",

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doi = "10.1007/s10529-008-9754-x",

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AU - Aquea, Felipe

AU - Poupin, María Josefina

AU - Matus, José Tomás

AU - Gebauer, Marlene

AU - Medina, Consuelo

AU - Arce-Johnson, Patricio

N1 - Funding Information: Acknowledgments F.A. and M.J.P was supported by a PhD fellowship from CONICYT. F.A was supported VRAID fellowship from the Pontificia Universidad Católica de Chile. J.T.M was supported by MECESUP.

PY - 2008/10

Y1 - 2008/10

N2 - Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.

AB - Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.

KW - Conifer embryogenesis

KW - Pinus radiata

KW - Somatic embryo

KW - Suspension culture

KW - Synthetic seed

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Synthetic seed production from somatic embryos of Pinus radiata

Abstract

Keywords

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