Synthetic seed production from somatic embryos of Pinus radiata

Felipe Aquea, María Josefina Poupin, José Tomás Matus, Marlene Gebauer, Consuelo Medina, Patricio Arce-Johnson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.

Original languageEnglish
Pages (from-to)1847-1852
Number of pages6
JournalBiotechnology Letters
Issue number10
StatePublished - Oct 2008


  • Conifer embryogenesis
  • Pinus radiata
  • Somatic embryo
  • Suspension culture
  • Synthetic seed


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