TY - JOUR
T1 - Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla
AU - Goldberg, Burt
AU - Goycoolea, Marcus V.
AU - Schleivert, Patrick M.
AU - Shea, Donald
AU - Schachern, Patricia
AU - Paparella, Michael M.
AU - Carpenter, Anna Maria
PY - 1981/8
Y1 - 1981/8
N2 - A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area of normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours. Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middle ear effusions and perillymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.
AB - A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area of normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours. Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middle ear effusions and perillymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.
UR - http://www.scopus.com/inward/record.url?scp=0019789784&partnerID=8YFLogxK
U2 - 10.1016/S0196-0709(81)80017-8
DO - 10.1016/S0196-0709(81)80017-8
M3 - Article
C2 - 7283064
AN - SCOPUS:0019789784
SN - 0196-0709
VL - 2
SP - 210
EP - 214
JO - American Journal of Otolaryngology--Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology--Head and Neck Medicine and Surgery
IS - 3
ER -