Optimisation of experimental conditions for synthesis of high affinity MIP nanoparticles

Sreenath Subrahmanyam; Antonio Guerreiro; Alessandro Poma; Ewa Moczko; Elena Piletska; Sergey Piletsky

doi:10.1016/j.eurpolymj.2012.09.022

Optimisation of experimental conditions for synthesis of high affinity MIP nanoparticles

Sreenath Subrahmanyam, Antonio Guerreiro, Alessandro Poma, Ewa Moczko, Elena Piletska, Sergey Piletsky

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Molecularly imprinted nanoparticles were synthesised via iniferter polymerisation in the presence of a chain terminator agent and at high monomer and template concentration. The amount of initiator, chain terminator agent and UV irradiation time were optimised in order to achieve maximum yield of discrete nanoparticles without causing macrogelation of the polymerisation mixture. Resultant particles were then purified from unreacted monomers via gel permeation and characterised by size-exclusion chromatography, dynamic light scattering and electron microscopy. The affinity towards the target was verified via surface plasmon resonance on immobilised templates. The yield of nanoparticles obtained using this approach was 14%, a significant increase when compared with similar methods using high monomer concentration and UV initiation.

Original language	English
Pages (from-to)	100-105
Number of pages	6
Journal	European Polymer Journal
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/j.eurpolymj.2012.09.022
State	Published - Jan 2013
Externally published	Yes

Keywords

Cocaine
Iniferter
Molecularly imprinted polymer
Nanoparticle

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10.1016/j.eurpolymj.2012.09.022

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abstract = "Molecularly imprinted nanoparticles were synthesised via iniferter polymerisation in the presence of a chain terminator agent and at high monomer and template concentration. The amount of initiator, chain terminator agent and UV irradiation time were optimised in order to achieve maximum yield of discrete nanoparticles without causing macrogelation of the polymerisation mixture. Resultant particles were then purified from unreacted monomers via gel permeation and characterised by size-exclusion chromatography, dynamic light scattering and electron microscopy. The affinity towards the target was verified via surface plasmon resonance on immobilised templates. The yield of nanoparticles obtained using this approach was 14%, a significant increase when compared with similar methods using high monomer concentration and UV initiation.",

keywords = "Cocaine, Iniferter, Molecularly imprinted polymer, Nanoparticle",

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AU - Subrahmanyam, Sreenath

AU - Guerreiro, Antonio

AU - Poma, Alessandro

AU - Moczko, Ewa

AU - Piletska, Elena

AU - Piletsky, Sergey

PY - 2013/1

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AB - Molecularly imprinted nanoparticles were synthesised via iniferter polymerisation in the presence of a chain terminator agent and at high monomer and template concentration. The amount of initiator, chain terminator agent and UV irradiation time were optimised in order to achieve maximum yield of discrete nanoparticles without causing macrogelation of the polymerisation mixture. Resultant particles were then purified from unreacted monomers via gel permeation and characterised by size-exclusion chromatography, dynamic light scattering and electron microscopy. The affinity towards the target was verified via surface plasmon resonance on immobilised templates. The yield of nanoparticles obtained using this approach was 14%, a significant increase when compared with similar methods using high monomer concentration and UV initiation.

KW - Cocaine

KW - Iniferter

KW - Molecularly imprinted polymer

KW - Nanoparticle

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Optimisation of experimental conditions for synthesis of high affinity MIP nanoparticles

Abstract

Keywords

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