Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante técnicas de biología molecular

Mónica Figueroa; Jorge Alonso-Gutiérrez; José Luis Campos; Ramón Méndez; Anuska Mosquera-Corral

Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante técnicas de biología molecular

Translated title of the contribution: Identification of microorganisms involved in nitrogen removal from wastewater treatment systems by means of molecular biology techniques

Mónica Figueroa, Jorge Alonso-Gutiérrez, José Luis Campos, Ramón Méndez, Anuska Mosquera-Corral

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The identification of the main bacteria populations present in the granular biomass from a biological reactor treating wastewater has been performed by applying two different molecular biology techniques. By means of the DGGE technique five different genera of heterotrophic bacteria (Thiothrix, Thauera, Cloroflexi, Coinamonas y Zoogloea) and one of ammonia oxidizing bacteria (Nitrosomonas) were identified. The FISH technique, based on microscopy, allowed the in situ visualization and quantification of those microorganisms. Special attention was paid to filamentous bacteria distribution (Thiothrix and Cloroflexi) which could exert a structural function in aerobic granular sludge.

Translated title of the contribution	Identification of microorganisms involved in nitrogen removal from wastewater treatment systems by means of molecular biology techniques
Original language	Spanish
Pages (from-to)	44-53
Number of pages	10
Journal	Tecnologia del Agua
Volume	30
Issue number	317
State	Published - Mar 2010
Externally published	Yes

Cite this

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title = "Identificaci{\'o}n de microorganismos implicados en la eliminaci{\'o}n de nitr{\'o}geno en sistemas de tratamiento de aguas residuales mediante t{\'e}cnicas de biolog{\'i}a molecular",

abstract = "The identification of the main bacteria populations present in the granular biomass from a biological reactor treating wastewater has been performed by applying two different molecular biology techniques. By means of the DGGE technique five different genera of heterotrophic bacteria (Thiothrix, Thauera, Cloroflexi, Coinamonas y Zoogloea) and one of ammonia oxidizing bacteria (Nitrosomonas) were identified. The FISH technique, based on microscopy, allowed the in situ visualization and quantification of those microorganisms. Special attention was paid to filamentous bacteria distribution (Thiothrix and Cloroflexi) which could exert a structural function in aerobic granular sludge.",

keywords = "16S, Aerobic granules, Ammonia oxidizing bacteria, DGGE, FISH, Filamentous bacteria, PCR, Sequencing",

author = "M{\'o}nica Figueroa and Jorge Alonso-Guti{\'e}rrez and Campos, {Jos{\'e} Luis} and Ram{\'o}n M{\'e}ndez and Anuska Mosquera-Corral",

year = "2010",

month = mar,

language = "Spanish",

volume = "30",

pages = "44--53",

journal = "Tecnologia del Agua",

issn = "0211-8173",

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Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante técnicas de biología molecular. / Figueroa, Mónica; Alonso-Gutiérrez, Jorge; Campos, José Luis et al.
In: Tecnologia del Agua, Vol. 30, No. 317, 03.2010, p. 44-53.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante técnicas de biología molecular

AU - Figueroa, Mónica

AU - Alonso-Gutiérrez, Jorge

AU - Campos, José Luis

AU - Méndez, Ramón

AU - Mosquera-Corral, Anuska

PY - 2010/3

Y1 - 2010/3

N2 - The identification of the main bacteria populations present in the granular biomass from a biological reactor treating wastewater has been performed by applying two different molecular biology techniques. By means of the DGGE technique five different genera of heterotrophic bacteria (Thiothrix, Thauera, Cloroflexi, Coinamonas y Zoogloea) and one of ammonia oxidizing bacteria (Nitrosomonas) were identified. The FISH technique, based on microscopy, allowed the in situ visualization and quantification of those microorganisms. Special attention was paid to filamentous bacteria distribution (Thiothrix and Cloroflexi) which could exert a structural function in aerobic granular sludge.

AB - The identification of the main bacteria populations present in the granular biomass from a biological reactor treating wastewater has been performed by applying two different molecular biology techniques. By means of the DGGE technique five different genera of heterotrophic bacteria (Thiothrix, Thauera, Cloroflexi, Coinamonas y Zoogloea) and one of ammonia oxidizing bacteria (Nitrosomonas) were identified. The FISH technique, based on microscopy, allowed the in situ visualization and quantification of those microorganisms. Special attention was paid to filamentous bacteria distribution (Thiothrix and Cloroflexi) which could exert a structural function in aerobic granular sludge.

KW - 16S

KW - Aerobic granules

KW - Ammonia oxidizing bacteria

KW - DGGE

KW - FISH

KW - Filamentous bacteria

KW - PCR

KW - Sequencing

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M3 - Article

AN - SCOPUS:77951776861

SN - 0211-8173

VL - 30

SP - 44

EP - 53

JO - Tecnologia del Agua

JF - Tecnologia del Agua

IS - 317

ER -

Identificación de microorganismos implicados en la eliminación de nitrógeno en sistemas de tratamiento de aguas residuales mediante técnicas de biología molecular

Abstract

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