Epitope approach in molecular imprinting of antibodies

Ewa Moczko, Antonio Guerreiro, César Cáceres, Elena Piletska, Börje Sellergren, Sergey A. Piletsky

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMIPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMIPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1124
DOIs
StatePublished - 15 Aug 2019
Externally publishedYes

Keywords

  • Affinity chromatography
  • Antibodies
  • Molecular imprinting polymers
  • Nanoparticles
  • Protein epitope
  • Solid-phase synthesis

Fingerprint

Dive into the research topics of 'Epitope approach in molecular imprinting of antibodies'. Together they form a unique fingerprint.

Cite this