TY - JOUR
T1 - Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine
AU - Dauelsberg, Patricia
AU - Matus, José Tomás
AU - Poupin, María Josefina
AU - Leiva-Ampuero, Andrés
AU - Godoy, Francisca
AU - Vega, Andrea
AU - Arce-Johnson, Patricio
N1 - Funding Information:
This work was supported by the Grapevine Breeding Project of the Technological Fruit Consortium , FONDECYT 1100709 and the Millennium Nucleus for Plant Functional Genomics (P06-009-F). P. Dauelsberg was supported by a PhD fellowship from VRAID, Pontificia Universidad Católica de Chile, and M.J. Poupin is supported by a Postdoctoral FONDECYT project (No. 3100040) and a CONICYT project (No. 79090016). We thank Michael Handford (University of Chile) for critically reading the manuscript.
PY - 2011/9/15
Y1 - 2011/9/15
N2 - In the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in Vitis vinifera were studied. Inflorescences were emasculated before capfall, immediately manually pollinated (EP) and fruit development was compared to emasculated but non-pollinated (ENP) and self-pollinated inflorescences (NESP). The diameter of berries derived from pollinated flowers (EP and NESP) was significantly higher than from non-pollinated flowers (ENP) at 21 days after emasculation/pollination (DAE), and a rapid increase in the size of the inner mesocarp, together with the presence of an embryo-like structure, were observed. The expression of gibberellin oxidases (GA20ox and GA2ox), anthranilate synthase (related to auxin synthesis) and cytokinin synthase coding genes was studied to assess the relationship between hormone synthesis and early berry development, while flower patterning genes were analyzed to describe floral transition. Significant expression changes were found for hormone-related genes, suggesting that their expression at early stages of berry development (13 DAE) is related to cell division and differentiation of mesocarp tissue at a later stage (21 DAE). Expression of hormone-related genes also correlates with the expression of VvHB13, a gene related to mesocarp expansion, and with an increased repression of floral patterning genes (PISTILLATA and TM6), which may contribute to prevent floral transition inhibiting fruit growth before fertilization takes place.
AB - In the present work, the effect of assisted fertilization on anatomical, morphological and gene expression changes occurring in carpels and during early stages of berry development in Vitis vinifera were studied. Inflorescences were emasculated before capfall, immediately manually pollinated (EP) and fruit development was compared to emasculated but non-pollinated (ENP) and self-pollinated inflorescences (NESP). The diameter of berries derived from pollinated flowers (EP and NESP) was significantly higher than from non-pollinated flowers (ENP) at 21 days after emasculation/pollination (DAE), and a rapid increase in the size of the inner mesocarp, together with the presence of an embryo-like structure, were observed. The expression of gibberellin oxidases (GA20ox and GA2ox), anthranilate synthase (related to auxin synthesis) and cytokinin synthase coding genes was studied to assess the relationship between hormone synthesis and early berry development, while flower patterning genes were analyzed to describe floral transition. Significant expression changes were found for hormone-related genes, suggesting that their expression at early stages of berry development (13 DAE) is related to cell division and differentiation of mesocarp tissue at a later stage (21 DAE). Expression of hormone-related genes also correlates with the expression of VvHB13, a gene related to mesocarp expansion, and with an increased repression of floral patterning genes (PISTILLATA and TM6), which may contribute to prevent floral transition inhibiting fruit growth before fertilization takes place.
KW - ASB1
KW - Anthesis
KW - Berry flesh
KW - Embryo development
KW - Flower patterning
KW - L1L
KW - Parthenocarpy
UR - http://www.scopus.com/inward/record.url?scp=79960312583&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2011.03.006
DO - 10.1016/j.jplph.2011.03.006
M3 - Article
C2 - 21497942
AN - SCOPUS:79960312583
SN - 0176-1617
VL - 168
SP - 1667
EP - 1674
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 14
ER -