TY - JOUR
T1 - Differential gene expression analysis in the scallop Argopecten purpuratus exposed to altered pH and temperature conditions in an upwelling-influenced farming area
AU - Benítez, Samanta
AU - Figueroa, Álvaro
AU - Lagos, Nelson A.
AU - Silva, Andrea X.
AU - Duarte, Cristian
AU - Vargas, Cristian A.
AU - Lardies, Marco A.
AU - Cárdenas, Leyla
N1 - Funding Information:
We acknowledge the continuous support received from Christian and Pamela Tapia at Invertec Ostimar Co. The authors thank E. Hormazábal for the scallop illustration. This study was supported by the Research Center Dynamics of High Latitude Marine Ecosystems (grant no. 15150003). SB acknowledges ANID doctoral scholarship (#21210012). AF the FONDECYT thank Fondecyt Postdoctorate N. 3190571. NAL, CAV and MAL acknowledge ANID–Millennium Science Initiative Program–Code ICN2019_015 (SECOS) and FONDECYT Grant N° 1190444, 1221322, and 1210171 (CAV). CAV also acknowledges support from ANID-Millennium Science Initiative Program-Code ICN12_019 (IMO).
Funding Information:
We acknowledge the continuous support received from Christian and Pamela Tapia at Invertec Ostimar Co. The authors thank E. Hormazábal for the scallop illustration. This study was supported by the Research Center Dynamics of High Latitude Marine Ecosystems (grant no. 15150003 ). SB acknowledges ANID doctoral scholarship (# 21210012 ). AF the FONDECYT thank Fondecyt Postdoctorate N. 3190571 . NAL, CAV and MAL acknowledge ANID–Millennium Science Initiative Program –Code ICN2019_015 (SECOS) and FONDECYT Grant N° 1190444 , 1221322 , and 1210171 (CAV). CAV also acknowledges support from ANID-Millennium Science Initiative Program -Code ICN12_019 (IMO).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2023/3
Y1 - 2023/3
N2 - Increased carbon dioxide in the atmosphere and its absorption across the ocean surface will alter natural variations in pH and temperature levels, occurring in coastal upwelling ecosystems. The scallop Argopecten purpuratus, one of the most economically important species farmed in northern Chile, has been shown to be vulnerable to these environmental drivers. However, the regulatory responses at the gene-level of scallops to these climate stressors remain almost unknown. Consequently, we used an orthogonal experimental design and RNAseq approach to analyze the acute effects of variability in pH and temperature on gene expression in the muscle tissue of A. purpuratus. In respect to control conditions (pH ~ 8.0/ 14 °C), the influence of low pH (~ 7.7) and temperature (14 °C) induced the activation of several genes associated with apoptotic signaling pathways and protein localization to plasma membrane. Elevated temperature (18 °C) and pH (~8.0) conditions increased the expression of transcripts associated with the activation of muscle contraction, regulation, and sarcomere organization effects on muscle tissue. In scallops exposed to low pH and elevated temperature, the genes expressed were differentially associated with the oxidation-reduction process, signal translation, and positive regulation of GTPase activity. These results indicated that the differentially expressed genes under the experimental conditions tested are mainly related to the mitigation of cellular damage and homeostasis control. Our results add knowledge about the function of the adductor muscle in response to stressors in scallops. Furthermore, these results could help in the identification of molecular biomarkers of stress necessary to be integrated into the aquaculture programs for the mitigation of climate change.
AB - Increased carbon dioxide in the atmosphere and its absorption across the ocean surface will alter natural variations in pH and temperature levels, occurring in coastal upwelling ecosystems. The scallop Argopecten purpuratus, one of the most economically important species farmed in northern Chile, has been shown to be vulnerable to these environmental drivers. However, the regulatory responses at the gene-level of scallops to these climate stressors remain almost unknown. Consequently, we used an orthogonal experimental design and RNAseq approach to analyze the acute effects of variability in pH and temperature on gene expression in the muscle tissue of A. purpuratus. In respect to control conditions (pH ~ 8.0/ 14 °C), the influence of low pH (~ 7.7) and temperature (14 °C) induced the activation of several genes associated with apoptotic signaling pathways and protein localization to plasma membrane. Elevated temperature (18 °C) and pH (~8.0) conditions increased the expression of transcripts associated with the activation of muscle contraction, regulation, and sarcomere organization effects on muscle tissue. In scallops exposed to low pH and elevated temperature, the genes expressed were differentially associated with the oxidation-reduction process, signal translation, and positive regulation of GTPase activity. These results indicated that the differentially expressed genes under the experimental conditions tested are mainly related to the mitigation of cellular damage and homeostasis control. Our results add knowledge about the function of the adductor muscle in response to stressors in scallops. Furthermore, these results could help in the identification of molecular biomarkers of stress necessary to be integrated into the aquaculture programs for the mitigation of climate change.
KW - Aquaculture
KW - Argopecten purpuratus
KW - Climate change
KW - RNAseq approach
KW - Upwelling
UR - http://www.scopus.com/inward/record.url?scp=85144092699&partnerID=8YFLogxK
U2 - 10.1016/j.cbd.2022.101046
DO - 10.1016/j.cbd.2022.101046
M3 - Article
C2 - 36495831
AN - SCOPUS:85144092699
SN - 1744-117X
VL - 45
JO - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
JF - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
M1 - 101046
ER -