TY - JOUR
T1 - Colorimetric determination of cysteamine based on the aggregation of polyvinylpyrrolidone-stabilized silver nanoparticles
AU - Shanmugaraj, Krishnamoorthy
AU - Sasikumar, Thangarasu
AU - Campos, Cristian H.
AU - Ilanchelian, Malaichamy
AU - Mangalaraja, Ramalinga Viswanathan
AU - Torres, Cecilia C.
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/8/5
Y1 - 2020/8/5
N2 - A simple, colorimetric and visual method is described for the determination of cysteamine (CA) using polyvinylpyrrolidone-stabilized silver nanoparticles (PVP-AgNPs) as a colorimetric probe. The sensing method was based on the aggregation of PVP-AgNPs that led to the changes in the color and absorption profile of the probe. The aggregation of PVP-AgNPs in the presence of CA was evidenced by using transmission electron microscopy (TEM), zeta and dynamic light scattering (DLS) measurements. A distinct color transition could be observed with the naked eye from pale yellow color of PVP-AgNPs to purple. PVP-AgNPs probe showed an excellent selectivity towards CA versus other interfering biomolecules, cations and anions. Furthermore, the colorimetric probe had a linear response for CA from 0.1 to 1.0 μM concentration range with the limit of detection (LOD) of 4.9 nM. The prepared probe was successfully utilized for the determination of CA in blood serum as biological samples.
AB - A simple, colorimetric and visual method is described for the determination of cysteamine (CA) using polyvinylpyrrolidone-stabilized silver nanoparticles (PVP-AgNPs) as a colorimetric probe. The sensing method was based on the aggregation of PVP-AgNPs that led to the changes in the color and absorption profile of the probe. The aggregation of PVP-AgNPs in the presence of CA was evidenced by using transmission electron microscopy (TEM), zeta and dynamic light scattering (DLS) measurements. A distinct color transition could be observed with the naked eye from pale yellow color of PVP-AgNPs to purple. PVP-AgNPs probe showed an excellent selectivity towards CA versus other interfering biomolecules, cations and anions. Furthermore, the colorimetric probe had a linear response for CA from 0.1 to 1.0 μM concentration range with the limit of detection (LOD) of 4.9 nM. The prepared probe was successfully utilized for the determination of CA in blood serum as biological samples.
KW - Aggregation
KW - Color change
KW - Cysteamine detection
KW - Optical probe
KW - Quantitative detection
KW - Visual detection
UR - http://www.scopus.com/inward/record.url?scp=85083668741&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2020.118281
DO - 10.1016/j.saa.2020.118281
M3 - Article
C2 - 32335419
AN - SCOPUS:85083668741
SN - 1386-1425
VL - 236
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
M1 - 118281
ER -