TY - JOUR
T1 - Binding of BAL 31 RNA polymerase to PM2 DNA as determined by electron microscopy and protection against restriction endonuclease cleavage.
AU - Bull, P.
AU - Susaeta, M.
AU - González, B.
AU - Yudelevich, A.
PY - 1988/10
Y1 - 1988/10
N2 - Specific binding sites of BAL 31 RNA polymerase on PM2 DNA have been mapped by protection against HincII and HindIII cleavage and by observation of enzyme-DNA complexes by electron microscopy. Nine specific binding sites were observed at map units 0.19, 0.20, 0.28, 0.54, 0.63, 0.65, 0.71, 0.72, and 0.75 by the first method. All these sites were confirmed by electron microscopy which, in addition, revealed another site at 0.05 map unit. Published nucleotide sequences of the region surrounding sites at 0.71 and 0.75 map units show the presence of consensus sequences for procaryotic promoters.
AB - Specific binding sites of BAL 31 RNA polymerase on PM2 DNA have been mapped by protection against HincII and HindIII cleavage and by observation of enzyme-DNA complexes by electron microscopy. Nine specific binding sites were observed at map units 0.19, 0.20, 0.28, 0.54, 0.63, 0.65, 0.71, 0.72, and 0.75 by the first method. All these sites were confirmed by electron microscopy which, in addition, revealed another site at 0.05 map unit. Published nucleotide sequences of the region surrounding sites at 0.71 and 0.75 map units show the presence of consensus sequences for procaryotic promoters.
UR - http://www.scopus.com/inward/record.url?scp=0024095275&partnerID=8YFLogxK
U2 - 10.1128/jvi.62.10.3911-3913.1988
DO - 10.1128/jvi.62.10.3911-3913.1988
M3 - Article
C2 - 2843687
AN - SCOPUS:0024095275
SN - 0022-538X
VL - 62
SP - 3911
EP - 3913
JO - Journal of virology
JF - Journal of virology
IS - 10
ER -