An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth

Ana Zúñiga; Francisco De La Fuente; Fernán Federici; Corinne Lionne; Jérome Bônnet; Victor De Lorenzo; Bernardo González

doi:10.1021/acssynbio.8b00002

An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth

Ana Zúñiga
, Francisco De La Fuente
, Fernán Federici
, Corinne Lionne
, Jérome Bônnet
, Victor De Lorenzo
, Bernardo González

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

Original language	English
Pages (from-to)	1519-1527
Number of pages	9
Journal	ACS Synthetic Biology
Volume	7
Issue number	6
DOIs	https://doi.org/10.1021/acssynbio.8b00002
State	Published - 15 Jun 2018
Externally published	Yes

Keywords

Arabidopsis thaliana
Cupriavidus pinatubonensis
quorum sensing
synthetic beneficial interaction

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10.1021/acssynbio.8b00002

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title = "An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth",

abstract = "The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.",

keywords = "Arabidopsis thaliana, Cupriavidus pinatubonensis, quorum sensing, synthetic beneficial interaction",

author = "Ana Z{\'u}{\~n}iga and Fuente, \{Francisco De La\} and Fern{\'a}n Federici and Corinne Lionne and J{\'e}rome B{\^o}nnet and \{De Lorenzo\}, Victor and Bernardo Gonz{\'a}lez",

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doi = "10.1021/acssynbio.8b00002",

language = "English",

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AU - Zúñiga, Ana

AU - Fuente, Francisco De La

AU - Federici, Fernán

AU - Lionne, Corinne

AU - Bônnet, Jérome

AU - De Lorenzo, Victor

AU - González, Bernardo

PY - 2018/6/15

Y1 - 2018/6/15

N2 - The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

AB - The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

KW - Arabidopsis thaliana

KW - Cupriavidus pinatubonensis

KW - quorum sensing

KW - synthetic beneficial interaction

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ER -

An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth

Abstract

Keywords

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