An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth

Ana Zúñiga, Francisco De La Fuente, Fernán Federici, Corinne Lionne, Jérome Bônnet, Victor De Lorenzo, Bernardo González

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

Original languageEnglish
Pages (from-to)1519-1527
Number of pages9
JournalACS Synthetic Biology
Volume7
Issue number6
DOIs
StatePublished - 15 Jun 2018
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Cupriavidus pinatubonensis
  • quorum sensing
  • synthetic beneficial interaction

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