TY - JOUR
T1 - An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 to Stimulate Plant Growth
AU - Zúñiga, Ana
AU - Fuente, Francisco De La
AU - Federici, Fernán
AU - Lionne, Corinne
AU - Bônnet, Jérome
AU - De Lorenzo, Victor
AU - González, Bernardo
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/6/15
Y1 - 2018/6/15
N2 - The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.
AB - The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.
KW - Arabidopsis thaliana
KW - Cupriavidus pinatubonensis
KW - quorum sensing
KW - synthetic beneficial interaction
UR - http://www.scopus.com/inward/record.url?scp=85046944755&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.8b00002
DO - 10.1021/acssynbio.8b00002
M3 - Article
C2 - 29746094
AN - SCOPUS:85046944755
SN - 2161-5063
VL - 7
SP - 1519
EP - 1527
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 6
ER -