A multispectroscopic and molecular docking investigation of the binding interaction between serum albumins and acid orange dye

Selvaraj Naveenraj, Rajadurai Vijay Solomon, Ramalinga Viswanathan Mangalaraja, Ponnambalam Venuvanalingam, Abdullah M. Asiri, Sambandam Anandan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The interaction of Acid Orange 10 (AO10) with bovine serum albumin (BSA) was investigated comparatively with that of human serum albumin (HSA) using multispectroscopic techniques for understanding their toxic mechanism. Further, density functional theory calculations and docking studies have been carried out to gain more insights into the nature of interactions existing between AO10 and serum albumins. The fluorescence results suggest that AO10 quenched the fluorescence of BSA through the combination of static and dynamic quenching mechanism. The same trend was followed in the interaction of AO10 with HSA. In addition to the type of quenching mechanism, the fluorescence spectroscopic results suggest that the binding occurs near the tryptophan moiety of serum albumins and the binding. AO10 has more binding affinity towards BSA than HSA. An AO10-Trp model has been created to explicitly understand the C–H[tbnd]π interactions from Bader's quantum theory of atoms in molecules analysis which confirmed that AO10 bind more strongly with BSA than that of HSA due to the formation of three hydrogen bonds with BSA whereas it forms two hydrogen bonds in the case of HSA. These obtained results provide an in-depth understanding of the interaction of the acid azo dye AO10 with serum albumins. This interaction study provides insights into the underlying reasons for toxicity of AO10 relevant to understand its effect on bovids and humans during the blood transportation process.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume192
DOIs
StatePublished - 5 Mar 2018
Externally publishedYes

Keywords

  • Azo dye
  • Dynamic quenching
  • Molecular modelling
  • QTAIM analysis
  • Serum albumins

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